Chromatography and its types

History[ edit ] The first on-line coupling of gas chromatography to a mass spectrometer was reported in InElectronic Associates, Inc.

Chromatography and its types

Drawing markers not permanent: Cut at least two strips, one to test brown and one to test yellow. Cut an extra strip for each additional color you would like to test.

How do you expect each of the different colors to behave when you test it with the paper strip? Draw a pencil line across the width of each paper strip, about one centimeter from the bottom end. Take the brown marker and a paper strip and draw a short line about one centimeter on the middle section of the pencil line.

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Your marker line should not touch the sides of your strip. Use a pencil to write the color of the marker you just used on the top end of the strip. Do not use the colored marker or pen to write on the strips, as the color or ink will run during the test.

Repeat the previous three steps with a yellow marker and then all the additional colors you would like to test. Hold a paper strip next to one of the tall glasses on the outside of italigning the top of the strip with the rim of the glass, then slowly add water to the glass until the level just reaches the bottom end of the paper strip.

Repeat with the other glass eskeeping the strips still on the outside and away from the water. What role do you think the water will play?

Procedure Fasten the top of a strip the side farthest from the marker line to the pencil with a binder clip or clothespin.

Pause for a moment. Do you expect this color to be the result of a mixture of colors or the result of one color molecule? If you like, you can make a note of your prediction now.

Hang the strip in one of the glasses that is partially filled with water by letting the pencil rest on the glass rim. The bottom end of the strip should just touch the water level. If needed, add water to the glass until it is just touching the paper.

It is important that the water level stays below the marker line on the strip.

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Leave the first strip in its glass as you repeat the previous two steps with the second strip and the second glass. Repeat with any additional colors you are testing. Watch as the water rises up the strips. What happens to the colored lines on the strips? Does the color run up as well?

Do you see any color separation? When the water level reaches about one centimeter from the top this may take up to 10 minutesremove the pencils with the strips attached from the glasses.

If you let the strips run too long, the water can reach the top of the strips and distort your results. Write down your observations.

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Did the colors run? Did they separate in different colors?Liquid Chromatography which has various abbreviations, the most common being HPLC, for high-performance liquid chromatography, the mobile phase is a liquid rather than a gas, often water or a simple organic compound, pumped through the stationary phase in a column under pressure.

Better for temperature-sensitive compounds and those that do .

Chromatography and its types

Description. The NIST 17 mass spectral library, the successor to the NIST 14, is a fully evaluated collection of electron ionization (EI) and MS/MS mass spectra, with chemical and GC data, plus search software to identify your own unknown spectra.

It is a product of a more than three decade, comprehensive evaluation and expansion of the world's most widely used mass spectral reference . Chromatography: Chromatography, technique for separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream, called the mobile phase, and a contiguous stationary phase.

Chromatography and its types

The mobile phase may . Scientific American is the essential guide to the most awe-inspiring advances in science and technology, explaining how they change our understanding of the world and shape our lives. 10 Types of Chromatography | Based on Different Techniques & Methods There are many types of chromatography like HPLC, Gas chromatography, paper chromatography, thin layer chromatography, etc.

Chromatography is an analytic technique which is based on the separation of molecules of a sample over two phases. Chromatography is a separation technique that every organic chemist and biochemist is familiar with. I, myself, being an organic chemist, have routinely carried out chromatographic separations of a variety of mixture of compounds in the lab.

3 Main Types of Chromatography Techniques (With Diagram)